RNA Isolation from Purified Rice Necrosis Mosaic Virus Particles

Main Article Content

Sopan Ganpatrao Wagh
Manoj Baliram Pohare
Abhijit Arun Daspute
Hanumant Baburao Wadekar
Sachin Ashok Bhor

Abstract

Rice, particularly grows in tropical and subtropical areas, is one of the most important crops worldwide. Rice necrosis mosaic virus (RNMV) was first reported in Japan and causes necrotic lesions on leaf and yellowing. This virus has been classified as member of the Potyviridae family's Bymovirus genus. The fungus, Polymixa graminis, transmits RNMV. In this study, the methodology first explained RNMV virus particle - purification by ultra-centrifugation along with sucrose and cesium chloride gradient is described. Further, confirmation of purified RNMV particles under the electron-microscope is discussed. In the last RNMV RNA isolation from the purified RNMV particles were explained.

Keywords:
Rice necrosis mosaic virus, purification, viral RNA isolation, bymovirus, rice.

Article Details

How to Cite
Wagh, S. G., Pohare, M. B., Daspute, A. A., Wadekar, H. B., & Bhor, S. A. (2020). RNA Isolation from Purified Rice Necrosis Mosaic Virus Particles. Archives of Current Research International, 19(3), 1-6. https://doi.org/10.9734/acri/2019/v19i330157
Section
Method Article

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