Advances in Fish Masculinization Techniques for Sustainable Aquaculture

Amisha Markam

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Dushyant Kumar Damle *

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Jham Lal *

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Priyanka Acharya

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Pranali Marbade

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Doman Nirmalkar

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Kriti Kumari

Fish Genetics & Biotechnology Division, ICAR-Central Institute of Fisheries Education, Mumbai-400061, India.

Jayashri S. Shelke

Fish Genetics & Biotechnology Division, ICAR-Central Institute of Fisheries Education, Mumbai-400061, India.

Reeya Mondal

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

Vijaylakshmi Sahu

Late Shri Punaram Nishad College of Fisheries, Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, Chhattisgarh-491995, India.

*Author to whom correspondence should be addressed.


Abstract

Fish masculinization in aquaculture refers to a process that involves the development of secondary male sexual characteristics in female fish or the alteration of the normal sexual differentiation process. This term refers to the production of male-typical characteristics. Methods of masculinization typically involve hormonal treatment, natural variation, genetic manipulation, and temperature control. 17α-Methyltestosterone is use for hormonal treatment it is a synthetic derivative of testosterone, which is a male sex hormone (androgen). Use medicinal herbs and plants like papaya seed (Carica papaya) for natural variations. They have active ingredients like caricaine, carpasemine enzyme, and oleanolic glycoside, which has been shown androgenic effect. Genetic manipulation is done by selective breeding or genetic alteration to create fish with male sex chromosomes. For temperature control, the temperature at which eggs are incubated can influence the sex of the offspring. Aromatase inhibitors are also used as masculinization agents. They work by blocking the aromatase enzyme, which turns the hormone androgen into small amounts of estrogen in the body. The benefits of masculinization include its potential to produce monosex populations, reduce the risk of breeding issues, and promote uniform growth.  However, challenges such as hormone-related side effects, environmental concerns, and long-term sustainability must be addressed. This study examines the methods and results of fish masculinization procedures in aquaculture, emphasizing their use, advantages, and difficulties for various species. It also makes recommendations for future research directions in the quest for more effective and sustainable aquaculture methods.

Keywords: Fish, masculinization, papaya seed, 17α-Methyltestosterone, aromatase inhibitor, sex-reversal


How to Cite

Amisha Markam, Dushyant Kumar Damle, Jham Lal, Priyanka Acharya, Pranali Marbade, Doman Nirmalkar, Kriti Kumari, Jayashri S. Shelke, Reeya Mondal, and Vijaylakshmi Sahu. 2025. “Advances in Fish Masculinization Techniques for Sustainable Aquaculture”. Archives of Current Research International 25 (9):381–396. https://doi.org/10.9734/acri/2025/v25i91504.