Archives of Current Research International

Green gram (Vigna radiata L. Wilczek) undergoes substantial biochemical and metabolic changes during germination that may influence its phytochemical composition and antioxidant properties. This study qualitatively profiled major phytochemical classes and quantified selected bioactive components in whole MGG 385 green gram pre-microgreens germinated for 48 h. Aqueous and 80% methanolic extracts were screened using complementary qualitative assays. Total phenols, total flavonoids, DPPH free-radical scavenging activity, ferric thiocyanate antioxidant activity and reducing power were also assessed. Carbohydrates, proteins and amino acids were detected more strongly in aqueous extracts, whereas methanolic extracts produced stronger reactions for phenolics, flavonoids, tannins, terpenoids and saponins. Alkaloids, cardiac glycosides, steroids and quinones were not detected under the experimental conditions. Germination increased total phenols from 9.02±0.11 to 19.53±0.40 mg GAE/100 g and total flavonoids from 13.17±0.81 to 24.53±2.37 mg RE/100 g. The DPPH IC₅₀ decreased from 196.67±0.58 to 16.21±0.45 µg/mL, while antioxidant activity and reducing power also increased in the germinated sample. These findings indicate that 48 h germination modified the extractable phytochemical profile and enhanced the measured antioxidant-related properties of MGG 385 green gram. Further chromatographic confirmation and evaluation of bioaccessibility and safety are required before food or nutraceutical applications are considered.