RNA Isolation from Purified Rice Necrosis Mosaic Virus Particles
Sopan Ganpatrao Wagh *
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan and Department of Bio-science and Technology, Agricultural Biotechnology College, VNMK University, Aurangabad 431003, MS, India.
Manoj Baliram Pohare
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Abhijit Arun Daspute
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Hanumant Baburao Wadekar
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan.
Sachin Ashok Bhor
The United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan.
*Author to whom correspondence should be addressed.
Abstract
Rice, particularly grows in tropical and subtropical areas, is one of the most important crops worldwide. Rice necrosis mosaic virus (RNMV) was first reported in Japan and causes necrotic lesions on leaf and yellowing. This virus has been classified as member of the Potyviridae family's Bymovirus genus. The fungus, Polymixa graminis, transmits RNMV. In this study, the methodology first explained RNMV virus particle - purification by ultra-centrifugation along with sucrose and cesium chloride gradient is described. Further, confirmation of purified RNMV particles under the electron-microscope is discussed. In the last RNMV RNA isolation from the purified RNMV particles were explained.
Keywords: Rice necrosis mosaic virus, purification, viral RNA isolation, bymovirus, rice.